goat anti catx Search Results


anti il 33  (R&D Systems)
95
R&D Systems anti il 33
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Anti Il 33, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
anti il 33 - by Bioz Stars, 2026-02
95/100 stars
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goat anti rabbit alexafluor 488  (Thermo Fisher)
86
Thermo Fisher goat anti rabbit alexafluor 488
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti Rabbit Alexafluor 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
goat anti rabbit alexafluor 488 - by Bioz Stars, 2026-02
86/100 stars
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alexa fluor 555 conjugated goat anti mouse igm antibody  (Thermo Fisher)
86
Thermo Fisher alexa fluor 555 conjugated goat anti mouse igm antibody
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Alexa Fluor 555 Conjugated Goat Anti Mouse Igm Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
alexa fluor 555 conjugated goat anti mouse igm antibody - by Bioz Stars, 2026-02
86/100 stars
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anti rabbit igg 680 lt  (LI-COR)
86
LI-COR anti rabbit igg 680 lt
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Anti Rabbit Igg 680 Lt, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
anti rabbit igg 680 lt - by Bioz Stars, 2026-02
86/100 stars
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hrp conjugated goat anti rabbit  (Advansta)
95
Advansta hrp conjugated goat anti rabbit
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Hrp Conjugated Goat Anti Rabbit, supplied by Advansta, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
hrp conjugated goat anti rabbit - by Bioz Stars, 2026-02
95/100 stars
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goat anti-mouse-hrp  (Becton Dickinson)
90
Becton Dickinson goat anti-mouse-hrp
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti Mouse Hrp, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
goat anti-mouse-hrp - by Bioz Stars, 2026-02
90/100 stars
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goat anti bat igg  (Bethyl)
93
Bethyl goat anti bat igg
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti Bat Igg, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti bat igg - by Bioz Stars, 2026-02
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goat anti v5 fitc conjugate  (Bethyl)
95
Bethyl goat anti v5 fitc conjugate
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti V5 Fitc Conjugate, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti v5 fitc conjugate - by Bioz Stars, 2026-02
95/100 stars
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goat anti human hsa ap  (Bethyl)
95
Bethyl goat anti human hsa ap
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti Human Hsa Ap, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
goat anti human hsa ap - by Bioz Stars, 2026-02
95/100 stars
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goat anti myc fitc conjugate  (Bethyl)
94
Bethyl goat anti myc fitc conjugate
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Goat Anti Myc Fitc Conjugate, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
goat anti myc fitc conjugate - by Bioz Stars, 2026-02
94/100 stars
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horseradish peroxidase hrp conjugated goat anti human igg secondary antibody  (Bethyl)
93
Bethyl horseradish peroxidase hrp conjugated goat anti human igg secondary antibody
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Horseradish Peroxidase Hrp Conjugated Goat Anti Human Igg Secondary Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
horseradish peroxidase hrp conjugated goat anti human igg secondary antibody - by Bioz Stars, 2026-02
93/100 stars
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anti human f ab 2 fragment  (Jackson Immuno)
93
Jackson Immuno anti human f ab 2 fragment
Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c <t>or</t> <t>IL-33</t> citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.
Anti Human F Ab 2 Fragment, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c or IL-33 citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-33 is a cell-intrinsic regulator of fitness during early B cell development.

doi: 10.4049/jimmunol.1900408

Figure Lengend Snippet: Bone marrow was isolated from the tibia and femur of naïve adult WT BALB/c or IL-33 citrine reporter mice (Il33cit/+) and analyzed by flow cytometry. (A) IL-33 citrine reporter expression in bone marrow CD19+ B cells. (B) IL-33 citrine reporter expression in bone marrow B cells by developmental subset; pro-B (B220+ IgM− CD19+ CD43+), large pre-B (LPB, B220+ IgM− CD19+ CD43− FSC-Ahi SSC-Ahi), small pre-B (SPB, B220+ IgM− CD19+ CD43− FSC-Alo SSC-Alo), and immature B cells (Imm, B220+ IgM+ CD23−). Grey histogram is WT; black line is Il33cit/+. (C) Percent citrine-expressing IL-33+ B cells from B. (D) Geometric MFI of IL-33 citrine in bone marrow B cells. (E) Il33 transcript levels by quantitative PCR normalized to Gapdh and compared by the ΔΔCt method in MACS-enriched, FACS-purified bone marrow B cells. (F) IL-33 protein by western blot in MACS-enriched, FACS-purified bone marrow B cells fractionated by nuclear and cytoplasmic protein from WT and Il33−/− mice. (G) Quantification of F by densitometry from multiple blots; pro-B and LPB are aggregated together and compared to aggregated SPB and Imm. LC = loading control. (H) IL-33 GFP reporter expression in bone marrow B cells by developmental subset in WT C57BL/6 (grey histogram) and Il33GFP/GFP mice (purple line). (I) Percent GFP-expressing IL-33+ cells from H. (J) Geometric MFI of IL-33 GFP in the marrow B cells. (K) IL-33 citrine or IL-33 GFP expression in bone marrow pre-pro-B cells (B220+ IgM− CD19− CD43+). Grey histogram is WT, black line is Il33cit/+, and purple line is Il33GFP/GFP. Data are combined from 2 (I) or 3 (C, E, and G) or representative of 2 (H, J, and K) or 3 (A, B, D, and F) independent experiments. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA with Tukey posttest or Mann-Whitney test; n.s. = not significant.

Article Snippet: Blots were incubated overnight at 4°C while shaking with the indicated primary antibodies: anti-IL-33 (R&D, Cat. #AF3626), anti-Lamin B1 (Abcam, Cat. #ab16048), or anti-Tubulin (Cell Signaling Technology, Cat. #3873).

Techniques: Isolation, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Purification, Western Blot, MANN-WHITNEY

Human bone marrow from healthy donors was collected and leukocytes were isolated for flow cytometric analysis with intracellular staining. (A) Representative intracellular staining for IL-33 in two donor samples by B cell subset compared to isotype control. Dashed line represents gating cutoff for IL-33hi cells. Pro-B (CD19+ CD10+ IgM− CD34+), large pre-B (LPB, CD19+ CD10+ IgM− CD34− FSC-Ahi SSC-Ahi), small pre-B (SPB, CD19+ CD10+ IgM− CD34− FSC-Alo SSC-Alo), immature B cells (Imm, CD19+ CD10+ CD34− IgM+ IgD−), and mature B cells including recirculating B cells (Mat, CD19+ CD10−). (B) Percentage of IL-33hi bone marrow B cells by subset. (C) Geometric MFI of IL-33 in bone marrow B cells by subset. (D) Normalized IL33 expression in B cell CLL (n=862) collected from peripheral blood as compared to normal peripheral blood CD19+ B cells (n=39) of healthy controls displayed as log2 fold change. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001) by one-way ANOVA with Tukey posttest or unpaired two tailed t-test with Welch’s correction.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-33 is a cell-intrinsic regulator of fitness during early B cell development.

doi: 10.4049/jimmunol.1900408

Figure Lengend Snippet: Human bone marrow from healthy donors was collected and leukocytes were isolated for flow cytometric analysis with intracellular staining. (A) Representative intracellular staining for IL-33 in two donor samples by B cell subset compared to isotype control. Dashed line represents gating cutoff for IL-33hi cells. Pro-B (CD19+ CD10+ IgM− CD34+), large pre-B (LPB, CD19+ CD10+ IgM− CD34− FSC-Ahi SSC-Ahi), small pre-B (SPB, CD19+ CD10+ IgM− CD34− FSC-Alo SSC-Alo), immature B cells (Imm, CD19+ CD10+ CD34− IgM+ IgD−), and mature B cells including recirculating B cells (Mat, CD19+ CD10−). (B) Percentage of IL-33hi bone marrow B cells by subset. (C) Geometric MFI of IL-33 in bone marrow B cells by subset. (D) Normalized IL33 expression in B cell CLL (n=862) collected from peripheral blood as compared to normal peripheral blood CD19+ B cells (n=39) of healthy controls displayed as log2 fold change. Data are displayed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001) by one-way ANOVA with Tukey posttest or unpaired two tailed t-test with Welch’s correction.

Article Snippet: Blots were incubated overnight at 4°C while shaking with the indicated primary antibodies: anti-IL-33 (R&D, Cat. #AF3626), anti-Lamin B1 (Abcam, Cat. #ab16048), or anti-Tubulin (Cell Signaling Technology, Cat. #3873).

Techniques: Isolation, Staining, Expressing, Two Tailed Test